Oral Presentation Australian Society for Medical Research Annual Scientific Meeting 2016

A Stromal Organiser Cell that Directs Neonatal Spleen Regeneration (#4)

Jonathan Tan 1 , Takeshi Watanabe 2
  1. Bond University, Robina, QLD, Australia
  2. The Tazuke Kofukai Medical Research Institute/Kitano Hospital, Osaka, Japan

Development of lymphoid tissue is determined by interactions between stromal lymphoid tissue organiser (LTo) and hematopoietic lymphoid tissue inducer (LTi) cells. A failure for LTo to receive appropriate activating signals during embryogenesis through lymphotoxin engagement leads to a complete cessation of lymph node (LN) and Peyer’s patch development, identifying LTo as a key stromal population for lymphoid tissue organogenesis. However, little is known about the equivalent stromal cells that induce spleen development. Here, by dissociating neonatal murine spleen stromal tissue for re-aggregation and transplant into adult mouse recipients, we have identified a MAdCAM-1+CD31+CD201+ spleen stromal organizer cell-type critical for new tissue formation. This finding provides an insight into the regulation of post-natal spleen tissue organogenesis, and could be exploited in the development of spleen regenerative therapies.