Phenotype studies have been widely used to identify and characterise splenic myeloid and dendritic cell (DC) subsets. However, these studies are limited by the availability of antibodies, the expression level of markers, and the specificity of markers. Multiple subsets can express the same marker, e.g. F4/80, a red pulp macrophage marker, is also expressed on monocytes and DC. In order to delineate the lineages of DC and tissue macrophages more completely, recent studies have employed transcriptome analysis to generate gene profiles unique to macrophages and DC, respectively (Gautier et al, 2012, Miller et al., 2012). A novel dendritic like cells (L-DC) described by Hey et al., 2016 resembles a myeloid cell on the basis of CD11bhiCD11cloCD43+MHCII-Ly6C- Ly6G-Siglec-F-CX3CR1lo phenotype, but a DC on the basis of antigen presenting function. To fully elucidate the relationship between L-DC, conventional DC (cDC) and myeloid subsets, transcriptome analysis were undertaken. Principal component analysis showed close grouping of monocytes, L-DC and cDC in the first principal component, but separation of L-DC and monocytes from cDC subsets in the second principal component. In addition, hierarchical clustering indicates a closer relationship between L-DC and resident monocytes, and then between these two subsets and inflammatory monocytes. A list of genes common to splenic cDC and monocytes were generated respectively. L-DC expressed most of the common cDC and common monocytes genes, suggesting L-DC appear to mirror both the cDC and monocytes lineage. Further analysis also showed upregulation of genes reflecting both DC and myeloid lineage when the L- DC gene profile was compared with the cDC profile. Lastly, comparison of gene profiles between L-DC and monocyte subsets, revealed CD300e as a distinct marker for L-DC.